The HDAC-Glo™ and SIRT-Glo™ Assays and Screening Systems provide a simple "Add-Mix-Measure" method that achieves maximum signal in as little as 15 minutes and is 10-to 100X more sensitive than comparable fluorescence-based systems.
HDAC-Glo™ I/II Assays
Measure the relative activity of HDAC class I and II enzymes from cell extracts or purified enzyme sources with these assays and screening systems.
Single-reagent-addition, homogeneous, luminescent assays and screening systems that measure the relative activity of the NAD+-dependent histone deacetylase class III enzymes (sirtuins; SIRTs) from purified enzyme sources.
Here we describe efforts to define the utility of the HDAC-Glo™ Assay using structurally diverse HDAC inhibitors and various cell lines.