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GoScript™ Reverse Transcription System

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors. GoScript™ Reverse Transcriptase is qualified for use in qPCR, including GoTaq® qPCR and Plexor® RT-qPCR systems.

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

GoScript™ Reverse Transcription System

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GoScript™ Reverse Transcription System

 Components

  • MgCl2

    A351B1 x 750μl

  • GoScript™ 5X Reaction Buffer

    A500B1 x 300μl

  • GoScript™ Reverse Transcriptase

    A501B1 x 50μl

  • Oligo(dT)15 Primer

    C110B1 x 50μg

  • PCR Nucleotide Mix

    C114G1 x 200μl

  • Random Primers

    C118B1 x 50μg

  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u

  • Nuclease-Free Water

    P119A1 x 1,250μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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 50 reactions
 - A5000 SEK 2.933,00 Add to cart

GoScript™ Reverse Transcription System

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GoScript™ Reverse Transcription System

 Components

  • MgCl2

    A351H1 x 1.2ml

  • GoScript™ 5X Reaction Buffer

    A500C1 x 600μl

  • GoScript™ Reverse Transcriptase

    A501C1 x 100μl

  • Oligo(dT)15 Primer

    C110B1 x 50μg

  • PCR Nucleotide Mix

    C114G1 x 200μl

  • Random Primers

    C118B1 x 50μg

  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u

  • Nuclease-Free Water

    P119A2 x 1,250μl
Close Window

  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Login to request to add this product to your Helix »


 100 reactions
 - A5001 SEK 5.663,00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

GoScript™ Reverse Transcriptase

Close Window

GoScript™ Reverse Transcriptase

 Components

  • MgCl2

    A351H1 x 1.2ml

  • GoScript™ 5X Reaction Buffer

    A500C1 x 600μl

  • GoScript™ Reverse Transcriptase

    A501C1 x 100μl
Close Window

  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Login to request to add this product to your Helix »


 100 reactions
 - A5003 SEK 3.084,00 Add to cart

GoScript™ Reverse Transcriptase

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GoScript™ Reverse Transcriptase

 Components

  • MgCl2

    A351H3 x 1.2ml

  • GoScript™ 5X Reaction Buffer

    A500D2 x 1.25ml

  • GoScript™ Reverse Transcriptase

    A501D1 x 500μl
Close Window

  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Login to request to add this product to your Helix »


 500 reactions
 - A5004 SEK 12.196,00 Add to cart

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Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Figures

Reverse transcription of an 8.9kb APC transcript.Figure 1. Reverse transcription of an 8.9kb APC transcript.

Human colon total RNA (Clontech Laboratories, Inc.) was reverse transcribed using oligo(dT) (O) or random primers (R) according to the manufacturer’s instructions for each reverse transcriptase. cDNA then was analyzed by endpoint PCR using GoTaq® Green Master Mix (Cat.# M7122) with primers for a 940bp amplicon at the 5´ end of the transcript. Each reaction (5µl) was analyzed by agarose gel electrophoresis and ethidium bromide staining. All samples shown were analyzed in the same data set and gel with the same scan settings. Gel images are representative of results from three separate experiments.

GoScript Reverse Transcriptase cDNA synthesis coupled with GoTaq qPCR Master Mix can provide detection of input templates over a 9-log order dynamic range.Figure 2. GoScript™ Reverse Transcriptase cDNA synthesis coupled with GoTaq® qPCR Master Mix can provide detection of input templates over a 9-log order dynamic range.

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Use Restrictions

A5000, A5001 For Research Use Only. Not for Use in Diagnostic Procedures.
A5003, A5004 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Patents - Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. No. 5,552,302.

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