The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots. The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods.
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ECL Western Blotting Substrate
Luminol Enhancer Solution
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This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.
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Store at 2–8°C.
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The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng) as quantitated via ELISA. Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with ECL Western Blotting Substrate (Cat.# W1015) and with 1-minute exposure.
W1001, W1015 For Research Use Only. Not for Use in Diagnostic Procedures.