GDNF Emax® Immunoassay System Technical Bulletin
Literature # TB221
The GDNF Emax® ImmunoAssay System is designed for the sensitive and specific detection of GDNF in an antibody sandwich format. In this format, 96-well plates are coated with Anti-GDNF Monoclonal Antibody (mAb), which binds soluble GDNF. The captured GDNF is bound by a second, specific polyclonal antibody (pAb). After washing, the amount of specifically bound pAb is detected using a species-specific antibody conjugated to horseradish peroxidase (HRP) as a tertiary reactant. The unbound conjugate is removed by washing, and following an incubation with a chromogenic substrate, the color change is measured. The amount of GDNF in the test solutions is proportional to the color generated in the oxidation-reduction reaction. Using this system, GDNF in tissue culture supernatants or tissue homogenates can be quantitated.
The GDNF Emax® ImmunoAssay System offers several benefits:
- Specificity: Specific detection of GDNF; typically less than 3% cross-reactivity with other neurotrophic factors at 10ng/ml.
- Sensitivity: Detects a minimum of 31.2pg/ml of GDNF.
- Flexibility: Available in sizes for two or five 96-well ELISA
plates; can configure plates as desired.
- High Value: Optimized reagents and protocol.
Printed in USA. Revised 9/09.