DPPIV-Glo™ Protease Assay Technical Bulletin

Literature # TB339

The DPPIV-Glo™ Protease Assay is a homogeneous, luminescent assay that measures dipeptidyl peptidase IV (DPPIV) activity. The DPPIV-Glo™ Protease Assay provides a proluminescent DPPIV substrate, Gly-Pro-aminoluciferin, in a buffer system optimized for DPPIV and luciferase activities. Addition of a single DPPIV-Glo™ Reagent in an "add-mix-measure" format results in DPPIV cleavage of the substrate and generation of a "glow-type" luminescent signal produced by the luciferase reaction. In this coupled-enzyme format, the signal is proportional to the amount of DPPIV activity present.