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Cancer Res. 69, 5768–5775. Long-lived Min mice develop advanced intestinal cancers through a genetically conservative pathway. 2009

Halberg, R.B., Waggoner, J., Rasmussen, K., White, A., Clipson, L., Prunuske, A.J., Bacher, J.W., Sullivan, R., Washington, M.K., Pitot, H.C., Petrini, J.H., Albertson, D.G. and Dove, W.F.

Notes: To better understand tumor progression in mice carrying the Min allele of Adenomatous polyposis coli (Apc), a longer lived cross was generated and studied. Intestinal tumors and adjacent normal tissue were microdissected, frozen in liquid nitrogen and genomic DNA isolated using the Magnesil® Genomic, Fixed Tissue System. The purified DNA was then used for microsatellite instability (MSI) analysis. (4067)

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Mol. Med. Reports Atenas 1, 123–129. HPV infection in Brazilian oral squamous cell carcinoma patients and its correlation with clinicopathological outcomes 2008

Oliveira, L.R., Ribeiro-Silva, A., Ramalho, L.N.Z., Simões, A.L. and Zucoloto, S.

Notes: In this study of the frequency of human papilloma virus (HPV) in patients with oral squamous cell carcinoma (OSCC), the MagneSil® Genomic, Fixed Tissue System was used to isolate DNA from formalin-fixed paraffin-embedded tissue samples from primary tumors and matched samples. Five microliters of genomic DNA was amplified using PCR Master Mix and primers for both a 110 bp fragment of human ß-globin gene and HPV genotype. After PCR, the product was analyzed on an 8% nondenaturing polyacrylamide gel and stained with AgNO3. (3938)

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J. Clin. Endocrinol. Metab. 91, 3826–3829. Primary hyperparathyroidism with a low-normal, atypical serum parathyroid hormone as shown by discordant immunoassay curves. 2006

Lafferty, F.W., Hamlin, C.R., Corrado, K.R., Arnold, A. and Shuck, J.M.

Notes: To compare the parathyroid hormone (PTH) gene from a human parathyroid adenoma sample to DNA from the same individual’s peripheral blood leukocytes, the tumor genomic DNA was purified from fixed, paraffin-embedded tissue using the MagneSil® Genomic, Fixed Tissue System. The isolated genomic DNA was then amplified using PCR primers for three exons and sequenced for analysis. (3557)

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Clin. Chem. 52, 2250–2257. Sensitive detection of KIT D816V in patients with mastocytosis. 2006

Tan, A., Westerman, D., McArthur, G.A., Lynch, K., Waring, P. and Dobrovic, A.

Notes: The authors wanted to develop a more sensitive assay to detect codon 816 pathogenic variations in people diagnosed with systemic mastocytosis. The Wizard® Genomic DNA Purification Kit was used to isolate DNA from peripheral blood and bone marrow aspirate samples. To extract DNA from 2–5 micron, paraffin-embedded samples of bone marrow trephine, skin, spleen or liver, the tissues were digested with Proteinase K for four days at 56°C prior to DNA purification using the Magnesil® Genomic Fixed Tissue System. The isolated DNA was subjected to two assays: enriched sequencing of mutant alleles (ESMA) after BsmAI restriction enzyme digestion, and allele-specific competitive blocker PCR (ACB-PCR). (3575)

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Mol. Hum. Reprod. 11, 833–836. A heterozygous mutation in the desert hedgehog gene in patients with mixed gonadal dysgenesis. 2005

Canto, P., Vilchis, F., Soderlund, D., Reyes, E. and Mendez, J.P.

Notes: In this study, coding sequence abnormalities in desert hedgehog (DHH), a gene involved in male sex differentiation, were investigated. Genomic DNA was isolated from paraffin-embedded gonadal tissue from individuals with mixed gonadal dysgenesis and from three controls using the MagneSil® Genomic, Fixed Tissue System. The purified DNA was then analyzed by exon-specific PCR, single-stranded conformation polymorphism (SSCP) analysis, and sequencing. (3447)

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J. Clin. Endocrinol. Metab. 89, 4480–4483. Mutations in the Desert hedgehog (DHH) gene in patients with 46,XY complete pure gonadal dysgenesis. 2004

Canto, P., Soderlund, D., Reyes, E. and Mendez J.P.

Notes: This paper describes use of the MagneSil® Genomic, Fixed Tissue System for purifyication of genomic DNA from paraffin-embedded gonadal tissues. The isolated genomic DNA was used in PCR to amplify a sequence from Desert hedgehog (DHH) Gene. The PCR products were used in sequencing reactions. (3179)

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