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Notes: Researchers used Promega’s cAMP-Dependent Protein Kinase Peptide Inhibitor to demonstrate that BAD kinase is phosphorylated through a cAMP-Dependent Protein Kinase (PKA) dependent pathway in Burkitt’s lymphoma BL-41 cells.  The PepTag® Non-Radioactive cAMP-Dependent Protein Kinase Assay was used to demonstrate PKA activity in BL-41 cell lysates.  In these experiments the researchers added cAMP with and without Promega’s cAMP-Dependent Protein Kinase Peptide Inhibitor (20μM) or Rp-cAMPS (100μM) to 0.2-2ug of BL-41 cell lysate.  Images of gels were depicted in the paper. (3134)

Notes: The Myristoylated Protein Kinase C Peptide Inhibitor and cAMP-Dependent Protein Kinase Peptide Inhibitor were used in cell and animal studies to help specifically identify Protein Kinase A and C activities. The researchers used a range of 0-12uM Myristoylated Protein Kinase C Peptide Inhibitor for the studies. Protein Kinase A and C activities were measured using the SignaTECT^® cAMP-Dependent Protein Kinase (PKA) Assay and SignaTECT^® Protein Kinase C (PKC) Assay Systems, respectively. Experiments were performed in human umbilical vein endothelial cells (HUVECs), bovine aortic endothelial cells, mouse corneas, and guinea pigs.  (3130)

Notes: Immunoprecipitates of CFTR become phosphorylated upon addition of a PKA catalytic subunit which is inhibitable by the Protein Kinase A Inhibitor Peptide. The SignaTECT^® cAMP-Dependent Protein Kinase Assay System was used to measure PKA activity in immunoprecipitates of the CFTR protein. The activity was almost totally inhibited by the Protein Kinase A Inhibitor Peptide. PKA activity was four times higher with the anti-CFTR antibody precipitates than with a control antibody and addition of cAMP increased the amount of phosphorylation observed in the immunoprecipitates. Immunoprecipitates were prepared from Calu-3 human airway epithelia cells. (0318)

Notes: The phosphorylation of rat Insulin Receptor 1 was investigated with cell lysates. The cAMP-Dependent Protein Kinase Peptide Inhibitor and Myristoylated Protein Kinase C Peptide Inhibitor were included in separate reactions to define which kinase was performing the phosphorylation. (0525)

Notes: This reference details use of the c-AMP-Dependent Protein Kinase Peptide Inhibitor. (0429)