Express Full-Length NanoLuc®-Kinase Fusions in Mammalian Cells
The PRKCE-NanoLuc® Fusion Vector contains the coding region of NanoLuc® luciferase fused to the C-terminus of human PRKCE kinase. It is designed for use with the NanoBRET® Target Engagement (TE) Intracellular Kinase Assay, Adherent Format protocol, where the plasmid can be transfected into various cell lines for PRKCE target engagement analysis. The NanoLuc® luciferase kinase fusion vectors are supplied transfection-ready. The CMV promoter drives the expression of the full-length NanoLuc® kinase fusion protein.
Vector Details:
About Kinase Fusion Vector Names
The placement of NanoLuc® either 5´ or 3´ of the kinase gene is optimized for use with NanoBRET® TE Assays and reflected in the vector name. For example, NanoLuc®-kinase implies that NanoLuc® is 5´ to the kinase gene (at the N-terminus of the kinase). Similarly, kinase-NanoLuc® implies NanoLuc® is 3´to the kinase gene (at the C-terminus of the kinase).
NanoBRET® Target Engagement (TE) Intracellular Kinase Assays
The NanoBRET® TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET® Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET® signal for estimating intracellular affinity against the target protein. The NanoBRET® Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET® Tracer assay is correct for each kinase fusion vector.
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