4-CORE® Buffer Pack
Aliquots of 10X Restriction Enzyme Buffers A, B, C and D
- Majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers
Catalog Number:
Size
Catalog Number: R9921
The 4-CORE® Buffer Pack contains convenient aliquots of Promega Restriction Enzyme 10X Buffers A, B, C and D. The majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers.
See the table of Promega 1X Restriction Enzyme Buffer Composition below.
1X Restriction Enzyme Buffer Composition.
| Buffer | pH (at 37°C) | Tris-HCl (mM) | MgCl2 (mM) | NaCl (mM) | KCl (mM) | DTT (mM) |
|---|---|---|---|---|---|---|
| A | 7.5 | 6 | 6 | 6 | — | 1 |
| B | 7.5 | 6 | 6 | 50 | — | 1 |
| C | 7.9 | 10 | 10 | 50 | — | 1 |
| D | 7.9 | 6 | 6 | 150 | — | 1 |
| E | 7.5 | 6 | 6 | 100 | — | 1 |
| F | 8.5 | 10 | 10 | 100 | — | 1 |
| G | 8.2 | 50 | 5 | — | — | — |
| H | 7.5 | 90 | 10 | 50 | — | — |
| J | 7.5 | 10 | 7 | — | 50 | 1 |
| K | 7.4 | 10 | 10 | — | 150 | 1 |
| L | 9.0 | 10 | 3 | 100 | — | — |
MULTI-CORE™ Buffer (1X) = 25mM Tris-acetate (pH 7.5 at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.
Notes:
- For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
- For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.
- All restriction enzymes are supplied with 10mg/ml Acetylated BSA. Although BSA is not absolutely required for activity, it has been shown to enhance activity of many restriction enzymes. We recommend adding BSA to all restriction digests at a final concentration of 0.1mg/ml.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Buffer A 10X Buffer |
R001A | 1 × 1ml |
|
Buffer B 10X Buffer |
R002A | 1 × 1ml |
|
Buffer C 10X Buffer |
R003A | 1 × 1ml |
|
Buffer D 10X Buffer |
R004A | 1 × 1ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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