MaxSpin® 96 System
Automation-Friendly System for Solid-Sample Incubation and Centrifugation
- Cat.# AS9040 contains 10 units of the MaxSpin® 96 System
- One unit of the MaxSpin® 96 System consists of 100 baskets, 1 frame, 1 U-shaped collar and 1 plate (96-well, deep-well)
- MaxSpin® 96 Plate footprint meets ANSI/SLAS standard dimensions
- Compatible with Maxprep® Liquid Handler preprocessing methods for Maxwell® FSC DNA IQ™ Casework Kit (Cat.# AS1550) and Maxwell® RSC Buccal Swab DNA Kit (Cat.# AS1640)
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Catalog Number: AS9040
Catalog Number: AS9010
Catalog Number: AS9020
Catalog Number: AS9030
Batch-Process Solid-Support Samples into a 96-Well Plate Format
The MaxSpin® 96 System is designed to allow extraction of biological material from a solid support into a 96-well plate format. The extraction transitions samples into an automation-friendly format that is easily adapted for use with a liquid-handling platform.
The MaxSpin® 96 System consists of the following plastic consumable components: 100 baskets, 1 frame, 1 U-shaped collar and 1 plate (2.0ml 96-well, deep-well). One MaxSpin® 96 Kit contains 10 units of the MaxSpin® 96 System. All components are manufactured in alignment with the ISO 18385 standard.
An individual solid support sample (e.g., buccal swab) is placed into a basket, which is inserted into the MaxSpin® 96 Frame stacked atop the deep-well plate. Up to 96 baskets holding 96 individual samples can be inserted into a single MaxSpin® 96 Frame, with each basket fitting into a single well of the deep-well plate beneath the frame. Extraction solution is added to each well, and then the plate is heat incubated to allow release of biological materials off the solid support and into the surrounding liquid solution. After incubation, the frame (along with baskets) is lifted, and the MaxSpin® 96 Collar is inserted between the frame and plate to raise the baskets above the level of extraction solution within the plate wells. The device is then centrifuged, allowing the liquid extractant to separate from the solid support by passing through the small holes at the bottom of each basket. The baskets, frame and collar are then discarded. The liquid extractant samples remaining in the MaxSpin® 96 Plate can be prepared for nucleic acid purification with an automated liquid handler platform.
Automated Solutions with Promega Chemistries and Instruments
The MaxSpin® 96 System is compatible with Promega extraction chemistries, including the Casework Direct System (Cat.# DC4560, DC4561) and Casework Extraction Kit (Cat.# DC6745) with the Maxwell® FSC DNA IQ™ Casework Kit (Cat.# AS1550) or the Maxwell® RSC Buccal Swab DNA Kit (Cat.# AS1640). The automated preparation of samples in the MaxSpin® 96 Kit for extraction is supported by methods on the Maxprep® Liquid Handler and Maxwell® Instruments.
Dimensions of the MaxSpin® 96 System
(with U-Shaped Collar inserted)
L: 13.0cm
W: 8.9cm
H: 6.1cm
Centrifuge Compatibility
The following centrifuge and rotor combinations have been confirmed as being compatible with the MaxSpin® 96 Kit (catalog numbers included where known):
| Centrifuge | Rotor |
|---|---|
| Eppendorf 5804, 5804 R, 5810 and 5810 R | A-2-DWP rotor (Cat.# 5804 740.009) with deepwell plate bucket (Cat.# 5804 743.008) |
| Beckman Coulter Allegra X-12 | SX4750 rotor and Microplate Carriers for SX4750 Rotor (Cat.# 393042) |
Known incompatible centrifuge and rotor combinations:
| Centrifuge | Rotor |
|---|---|
| Eppendorf 5804, 5804 R, 5810 and 5810 R | A-4-62 rotor with 4 MTP buckets (Cat.# 5810 711.002) |
| Thermo IEC Centra CL3 centrifuge | Thermo IEC Centra CL3 centrifuge |
Protocols
Complete Protocol
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