β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
Colorimetric Detection of β-Gal Activity
- Safe, non-isotopic assay
- Can be used in 96-well plate format
- Reporter Lysis Buffer allows luciferase, CAT and β-gal assays to be performed from the same cell extract
Catalog Number:
Size
Catalog Number: E2000
Simple Detection of β-Gal Activity in Bacterial and Mammalian Cell Lysates
The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as the pSV-β-Galactosidase Control Vector.
The standard assay is performed by adding a dilute sample to an equal volume of Assay 2X Buffer that contains the substrate ONPG (o-nitrophenyl-β-d-galactopyranoside). Samples are incubated for at least 30 minutes, during which time the β-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl, which is yellow. The reaction can be terminated by addition of sodium carbonate, and the absorbance at 420nm is measured by spectrophotometry.
Cat.# E2000 contains sufficient reagents for 65 standard assays or 200 assays in a 96-well plate format.
References- Miller, J.H., ed. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
- Sambrook, J. et al. (1989) Molecular Cloning, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
- Schenborn, E. and Goiffon, V. (1993) Promega Notes 41, 11–3.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Sodium Carbonate |
E202A | 1 × 35ml | 1M | |
|
Assay 2X Buffer |
E203A | 1 × 10ml | ||
|
Reporter Lysis 5X Buffer |
E397A | 1 × 30ml | View Product | |
|
β-Galactosidase |
E801A | 1 × 100u |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
Resources
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