The Wizard® DNA Clean-Up System provides a simple and effective way to purify linear and circular DNA (200–50,000bp) from many molecular biology reactions:
- restriction enzymes, including heat-stable restriction enzymes
- phosphatases and kinases
- DNA polymerases, including Taq DNA polymerase
- exonucleases and endonucleases, including DNase I but not RNases
- mononucleotides
- salts
Using a quick batch-column procedure, the entire process can be completed in 15 minutes or less with no organic extractions or ethanol precipitations. DNA is eluted in water or TE buffer, ready for use. Multiple preps may be processed easily at one time with the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231).
This product requires plungers (not provided) for use with the purification protocol without a vacuum manifold.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® Minicolumns |
A721B | 2 × 50 each | View Product |
|
Wizard® DNA Clean-Up Resin |
A731A | 1 × 100ml | |
|
Syringe Barrels, 3cc |
A809B | 2 × 50 pack |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 5,808,041.
Resources
No related resources available
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Frequently Used With
Molecular Biology Lab Guide
A resource designed for scientists just embarking on their career, focusing on fundamental technologies and techniques.
GEN1978
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A7231
Vac-Man® Jr. Laboratory Vacuum Manifold
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