Viability PCR Reagent System Technical Manual
Instructions for Use of Product(s)
A8881, A8883
Literature # TM736
The Viability PCR Reagent System combines the rapid results of PCR and the ability to discriminate between signal from viable and nonviable cells and viruses. This kit contains a cell- and capsid-impermeable intercalating reagent that exclusively binds to nucleic acid from membrane- or capsid-compromised nonviable cells and viruses. Once bound, the molecule irreversibly and covalently modifies nucleic acid, making it nonamplifiable in PCR. As a result, the signal from the PCR detection reaction results almost exclusively from viable cells or viruses.
Summary of Changes
The following change was made to the 8/26 revision of this document:
1. Updated Section 6, Troubleshooting.
Revised 8/26.